The EcoRI restriction endonuclease with bacteriophage λ DNA. Equilibrium binding studies
نویسندگان
چکیده
منابع مشابه
The EcoRI restriction endonuclease with bacteriophage I DNA Equilibrium binding studies
The EcoRI restriction endonuclease was found by the filter binding technique to form stable complexes, in the absence of Mg2+, with the DNA from derivatives of bacteriophage A that either contain or lack EcoRI recognition sites. The amount of complex formed at different enzyme concentrations followed a hyperbolic equilibrium-binding curve with DNA molecules containing EcoRI recognition sites, b...
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The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA hexanucleotide sequence d(N-G-A-A-T-T-C-N)/d(N-C-T-T-A-A-G-N) when the ionic strength is high, 100 mM Tris-HCl, 50 mM NaCl, 5 mM MgCl2, and the pH is approximately 7.3. Lowering the ionic strength to 25 ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1980
ISSN: 0264-6021
DOI: 10.1042/bj1910593